A Triple-Fluorophore-Labeled Nucleic Acid pH Nanosensor to Investigate Non-viral Gene Delivery

A Triple-Fluorophore-Labeled Nucleic Acid pH Nanosensor to Investigate Non-viral Gene Delivery.

There is a need for new tools to better quantify intracellular delivery barriers in high-throughput and high-content ways. Here, we synthesized a triple-fluorophore-labeled nucleic acid pH nanosensor for measuring intracellular pH of exogenous DNA at specific time points in a high-throughput manner by flow cytometry following non-viral transfection. By including two pH-sensitive fluorophores an...

Endosomal Escape Pathways for Non-Viral Nucleic Acid Delivery Systems

Non-Viral Nucleic Acid Delivery Strategies to the Central Nervous System

With an increased prevalence and understanding of central nervous system (CNS) injuries and neurological disorders, nucleic acid therapies are gaining promise as a way to regenerate lost neurons or halt disease progression. While more viral vectors have been used clinically as tools for gene delivery, non-viral vectors are gaining interest due to lower safety concerns and the ability to deliver...

Nucleic acid carriers for pulmonary gene delivery

The delivery of nucleic acids to the pulmonary route appears to be promising due to non-invasiveness, large area of epithelial surface lining the lung, easy accessibility and ability to provide a platform for local delivery. The therapeutic role of nucleic acids in various diseases pertaining to the pulmonary route e.g. cystic fibrosis, α1-antitrypsin deficiency, asthma, chronic obstructive pul...

Recent progress in non-viral nucleic acids delivery.

A major goal of nucleic acid based therapies is to treat inherted and acquired disorders by adding, correcting, suppressing or eplacing genes (Abbas et al., 2008). Advantages of non-viral vectors or delivering nucleic acid based therapies include ease of scale-up, torage stability and improved quality control. The most promisng non-viral vectors have been liposomes and cationic polymers hich co...